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Department of Glycoconjugate Biochemistry Faculty of Biology and Earth Sciences Jagiellonian University
keywords: environment, glycosylation changes, cytotoxicity tests, SDS-PAGE electrophoresis
offer number: 12
research topics:
- analyses of glycosylation of cancer cell and glycoproteins from body fluids (saliva, blood);
- analyses of expression of glycosylotransferases at protein and nucleic acid levels, alterations of glycosylation occurring during various diseases (especially cancer), after administration of drugs (e.g. drugs used in immunosuppressive therapy) and after silencing or overexpression of particular proteins;
- an application of different synthetic chemicals (e.g. cis-platin, dacarbazine) and natural compounds to induce apoptosis of tumour cells;
- effects of various compounds on cell proliferation, viability, tumour migration and adhesion properties (e.g. adrenaline, estrogens).
test equipment:
- laminar air flow chamber (Nu-440-400E, USA, 2011);
- incubator CO2 (Lab-Line Instruments 490-1CE, USA, 2001);
- inverted microscope (Axiovert 40 CFL, Zeiss, Germany, 2008);
- automated cell counter (Bio-Rad Laboratories, 2010);
- autoclave (SterilClave 18BHD, Cominox, 2010);
- laminar chamber (ESCO APC-2A1, Singapur, 2003);
- thermal cycler (MJ Research PTC-200, USA, 1997);
- Real Time PCR (StepOne Plus, Applied Biosystems, Singapur, 2005);
- nucleic acids electrophoresis equipment Bio-Rad and Sigma Aldrich (Bio-Rad Laboratories 2003; Sigma, 2004);
- transilluminator (Uvitec, 2002);
- SDS-PAGE and WB equipment, Bio-Rad and Sigma Aldrich (Bio-Rad Laboratories, 2003; Sigma, 2004);
- GeneGnome Bio Imaging System for Chemiluminescence (Syngene, 2012);
- FreeZone Freeze Dry Systems (Labconco, USA, 2003);
- flow cytometer (Becton Dickinson FACS Calibur, 2005);
- centrifuges: MPW 350R (2005),Hettich 22R (2002), Labofuge 400R (2006).
comercial research:
- in possession of several dozens of melanoma cell lines derived from different stages of tumor progression that can serve as models for the assessment of cytotoxicity and in studies of protein expression (at mRNA, protein and oligosaccharide levels) under the influence of chemical compounds (e.g. drugs, cosmetic ingredients), biological or physical properties (such as UV radiation, temperature, humidity);
- the possibility to perform analyses of protein expression (at mRNA, protein and sugar component levels) in human or animal specimens (e.g. body fluids, tissue sections, biopsy material);
- cytotoxicity tests of different compounds on cell lines cultured in vitro;
- analyses of glycosylation changes (cell glycoproteins and proteins isolated from body fluids) under an influence of various compounds and during various diseases;
- analyses of cell properties such as adhesion and migration.
Methods and research techniques:
- SDS-PAGE electrophoresis and WB;
- flow cytometry;
- confocal microscopy;
- PCR and real-time PCR;
- transfection of cells;
- in vitro cell culture.
variants of cooperation: contract research, project, consortium
type of services: analysis, devices for rent, expertises
information / broker of Jagiellonian University
name and surname:
phone number: + 48 12 664 42 00
email: cittru@uj.edu.pl